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Interest in craft beers is increasing worldwide due to their flavor and variety. However, craft breweries have high water, energy, and carbon dioxide (CO2) demands and generate large quantities of high-strength waste and greenhouse gases. While many large breweries recover energy using anaerobic digestion (AD) and recapture CO2 from beer fermentation, little is known about the economic feasibility of applying these technologies at the scale of small craft breweries. In addition, compounds in hops (Humulus lupulus), which are commonly added to craft beer to provide a bitter or “hoppy” flavor, have been shown to adversely affect anaerobic microbes in ruminant studies. In this study, biochemical methane potential (BMP) assays and anaerobic sequencing batch reactor (ASBR) studies were used to investigate biomethane production from high-strength craft brewery waste, with and without hop addition. A spreadsheet tool was developed to evaluate the economic feasibility of bioenergy and CO2 recovery depending on the brewery’s location, production volume, waste management, CO2 requirement, energy costs, and hop waste addition. The results showed that co-digestion of yeast waste with 20% hops (based on chemical oxygen demand (COD)) resulted in slightly lower methane yields compared with mono-digestion of yeast; however, it did not significantly impact the economic feasibility of AD in craft breweries. The use of AD and CO2 recovery was found to be economically feasible if the brewery’s annual beer production is >50,000 barrels/year. 

ZigBee is a popular wireless communication standard for Internet of Things (IoT) networks. Since each ZigBee network uses hop-by-hop network-layer message authentication based Yanchao Zhang Arizona State University Star E E Tree E E R E Mesh E E R E E E on a common network key, it is highly vulnerable to packetC E injection attacks, in which the adversary exploits the compromised network key to inject arbitrary fake packets from any spoofed address to disrupt network operations and conCoordinator C R E sume the network/device resources. In this paper, we present PhyAuth, a PHY hop-by-hop message authentication frameE E C R R E E E R R C R E E Router E E E End Device Figure 1: ZigBee network topologies. work to defend against packet-injection attacks in ZigBee networks. The key idea of PhyAuth is to let each ZigBee E The coordinator acts as a central node responsible for mantransmitter embed into its PHY signals a PHY one-time password (called POTP) derived from a device-specific secret key and an efficient cryptographic hash function. An authentic POTP serves as the transmitter’s PHY transmission permission for the corresponding packet. PhyAuth provides three schemes to embed, detect, and verify POTPs based on different features of ZigBee PHY signals. In addition, PhyAuth involves lightweight PHY signal processing and no change to the ZigBee protocolstack. Comprehensive USRP experiments confirm that PhyAuth can efficiently detect fake packets with very low false-positive and false-negative rates while having a negligible negative impact on normal data transmissions. 

Commodity ultra-high-frequency (UHF) RFID authentication systems only provide weak user authentication, as RFID tags can be easily stolen, lost, or cloned by attackers. This paper presents the design and evaluation of SmartRFID, a novel UHF RFID authentication system to promote commodity crypto-less UHF RFID tags for security-sensitive applications. SmartRFID explores extremely popular smart devices and requires a legitimate user to enroll his smart device along with his RFID tag. Besides authenticating the RFID tag as usual, SmartRFID verifies whether the user simultaneously possesses the associated smart device with both feature-based machine learning and deep learning techniques. The user is considered authentic if and only if passing the dual verifications. Comprehensive user experiments on commodity smartwatches and RFID devices confirmed the high security and usability of SmartRFID. In particular, SmartRFID achieves a true acceptance rate of above 97.5% and a false acceptance rate of less than 0.7% based on deep learning. In addition, SmartRFID can achieve an average authentication latency of less than 2.21s, which is comparable to inputting a PIN on a door keypad or smartphone. 

Recent research has elucidated mechanochemical pathways of single cell polarization, but much less is known about collective motility initiation in adhesive cell groups. We used galvanotactic assays of zebrafish keratocyte cell groups, pharmacological perturbations, electric field switches, particle imaging velocimetry, and cell tracking to show that large cell groups initiate motility in minutes toward the cathode. Interestingly, while PI3K-inhibited single cells are biased toward the anode, inhibiting PI3K does not affect the cathode-directed cell group migration. We observed that control groups had the fastest cathode-migrating cell at the front, while the front cells in PI3K-inhibited groups were the slowest. Both control and PI3K-inhibited groups rapidly repolarized when the electric field direction was reversed, and the group migration continued after the electric field was switched off. Inhibiting myosin disrupted the cohesiveness of keratocyte groups and abolished the collective directionality and ability to switch direction when the electric field is reversed. Our data are consistent with a model according to which cells in the group sense the electric field individually and mechanical integration of the cells results in coherent group motility. 

Abstract. Despite offsetting global mean surface temperature, various studies demonstrated that stratospheric aerosol injection (SAI) could influence the recovery of stratospheric ozone and have important impacts on stratospheric and tropospheric circulation, thereby potentially playing an important role in modulating regional and seasonal climate variability. However, so far, most of the assessments of such an approach have come from climate model simulations in which SO2 is injected only in a single location or a set of locations. Here we use CESM2-WACCM6 SAI simulations under a comprehensive set of SAI strategies achieving the same global mean surface temperature with different locations and/or timing of injections, namely an equatorial injection, an annual injection of equal amounts of SO2 at 15∘ N and 15∘ S, an annual injection of equal amounts of SO2 at 30∘ N and 30∘ S, and a polar strategy injecting SO2 at 60∘ N and 60∘ S only in spring in each hemisphere. We demonstrate that despite achieving the same global mean surface temperature, the different strategies result in contrastingly different magnitudes of the aerosol-induced lower stratospheric warming, stratospheric moistening, strengthening of stratospheric polar jets in both hemispheres, and changes in the speed of the residual circulation. These impacts tend to maximise under the equatorial injection strategy and become smaller as the aerosols are injected away from the Equator into the subtropics and higher latitudes. In conjunction with the differences in direct radiative impacts at the surface, these different stratospheric changes drive different impacts on the extratropical modes of variability (Northern and Southern Annular modes), including important consequences on the northern winter surface climate, and on the intensity of tropical tropospheric Walker and Hadley circulations, which drive tropical precipitation patterns. Finally, we demonstrate that the choice of injection strategy also plays a first-order role in the future evolution of stratospheric ozone under SAI throughout the globe. Overall, our results contribute to an increased understanding of the fine interplay of various radiative, dynamical, and chemical processes driving the atmospheric circulation and ozone response to SAI and lay the foundation for designing an optimal SAI strategy that could form a basis of future multi-model intercomparisons.

 

A silicon integrated microfluidics system prints picoliter-segmented analytes for attomole-level chemical analysis with mass spectrometry imaging.